AA0100

Sigma

 

Ammonia Assay Kit

sufficient for 100 assays


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Description

Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.
ApplicationThis kit is for the quantitative, enzymatic determination of ammonia in food and biological samples. Ammonia reacts with α-ketoglutaric acid (KGA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of L-glutamate dehydrogenase (GlDH) to form L-glutamate and oxidized nicotinamide adenine dinucleotide phosphate (NADP+). The decrease in absorbance at 340 nm, due to the oxidation of NADPH, is proportional to the ammonia concentration. L-Glutamate dehydrogenase reacts specifically with ammonia. The Ammonia Assay Kit may be used to determine ammonia concentrations in the range of 0.2-15 μg/ml.

Properties

usage sufficient for 100 assays
storage temp.2-8°C

Safety

Hazard CodesXi
Risk Statements36/37/38
Safety Statements26-36/37

References

referenceBergmeyer, H.U., and Beutler, H-O. Meth. Enzym. Anal. 3rd ed., New York, NY , 454-461, (1985)
 Neeley, W.E., and Phillipson, J., Automated Enzymatic Method for Determining Ammonia in Plasma. Clin. Chem. 34, 1868-1869, (1988) Abstract

Components

  Kit component only
Description
Ammonia Assay Reagent 10 vials
L-Glutamate Dehydrogenase for Ammonia Assay 1 vial
Ammonia Standard Solution 1 vial